Trackit 1 kb plus dna ladder thermo fisher scientific. Feb 15, 2012 in a 1 kb ladder, the bands are 1kb apart in size, though ones at the lower end may be closer in size than 1kb. Sufficient plasmid dna can be isolated from 100 ml e. The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Pdf dna ladder is commonly used to determine the size of dna fragments by electrophoresis in. Use the loading dye solutions supplied with every fermentas dna ladder. Product information thermo scientific generuler 1 kb dna. Material safety data sheet full scale dna ladder, 1kb. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. For optimal resolution, the recommended agarose gel concentration is 0.
The digested dna includes fragments ranging from 0. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. The 1 kb and 3 kb bands contain more dna to provide internal orientation. If samples need to be diluted, use te or other buffer of minimal ionic strength. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and. This product is available in quickload purple format enjoy the convenience of a readytoload format. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restrict. Please have a look into the related application note for more information about ladder migration in different gel concentrations. Generuler 1 kb plus dna ladder 1250 25 x 50 g for 2500 applications, 0.
The following file naming structure is used to name the majority of these document files. Thermo scientific generuler 1 kb dna ladder, readytouse, is recommended for sizing and approximate quantification of a wide range of doublestranded dna on agarose gels. Use the loading dye solutions supplied with every fermentas dna laddermarker, as these solutions contain equilibrated amount of tracking dyes which will not mask dna under uv light. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. Description thermo scientific generuler 1 kb dna ladder is.
Generuler 1kb dna ladder, range 250 to 10,000bpcan be labeled radioactively with t4 polynucleotide kinase seeprotocol. This marker can be used with either agarose or polyacrylamide gels. The ladder is composed of fifteen chromatographypurified. Thermo scientific generuler 1 kb dna ladder 1kb dna ladder. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Pdf creator software latest version free download 16rqrz pdf creator software latest version free download 3d39b66ab9. Thermo scientific generuler 1 kb dna ladder, readytouse. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp.
Contents amount storage sm31 generuler 1 kb plus dna ladder 250 5 x 50 g for 500 applications, 0. Hyperladder 1kb is our most popular molecular weight marker, composed of a restriction digest plus one or more pcr products, especially designed for easy. While neb develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be. Unless otherwise indicated, 200 nm of each strand was assembled in 0. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. Hyperladder 1kb with 1 % wv agarose gel, hyperladder 50bp with 1. If the ionic strength of the sample is too low, blurring of the bands can occur. How can i prepare working solution with 1 kb dna ladder. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. William smith general phone 2755731 molecular biology sales specialist.
Quality control tests are performed on each new lot of neb product to meet the specifications designated for it. Pdf straightforward procedure for laboratory production of dna. U of rochester biological supply ctr g8633 stock manager. Thermo scientific ogeneruler 1 kb dna ladder, readyto. Generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of doublestranded dna ranging from 10 bp to 48. A dna ladder is a solution of dna molecules of different. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide range doublestranded dna on. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification.
Dna ladder 100 bp and dna ladder 1kb marker for dna rna agarose gel electrophoresis. This 1kb dna ladder is a convenient marker for determining the size of doublestranded dna from 250 to 10,000 base pairs. The dna ladder consists of proprietary plasmids which. For your convieniance there is a picture of the ladder presented here, but you should go to the fermentas site to read up on this ladder. The ladder is a mixture of chromatographypurified individual dna fragments. By selecting the generuler dna ladder of interest in table 1, you will be directed to the corresponding product page for more details and ordering information. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Midranger 1kb dna ladder 300bp 5, 000bp catalogue number. The product is supplied in a solution of 10 mm trishcl, ph 8. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Load the same volumes of the dna sample and the dna ladder. Thermo scientific ogeneruler 1 kb dna ladder, readytouse 25010,000 bp perform sizing and approximate quantification of a wide range of doublestranded dna on agarose gels with this 1 kb dna ladder.
The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. The ladder is supplied with 6x dna loading dye, and it can be used in dna sizing and approximate quanitification applications. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Contents amount storage sm03 generuler 1 kb dna ladder, readytouse 250 5 x 50 g for 500 applications, 0. The ladder is composed of fourteen chromatographypurified. Please have a look into the related application note for more information about ladder. Pdf straightforward procedure for laboratory production. The ladder consists of fragments ranging in size from 250 to 10,000 base pairs. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. High quality readytouse doublestranded dna ladder derived by recombinant.
Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the product specification sheet, certificate of analysis, data card or product manual. Highranger 1kb dna ladder 300bp 10,000bp catalogue number. Do not heat the 1kb plus dna ladder before loading. Generuler 1 kb dna ladder thermo fisher scientific. Do not exceed the amount of electrophoresis tracking dyes used for sampleladder preparation. The ladder is premixed with gel load buffer color dyes with. Straightforward procedure for laboratory production of dna ladder. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. Generuler 1 kb plus dna ladder catalog number sm31, sm32 pub. You really need to check the technical info that comes with the ladder for the details on each product, as some have different product names which mean the same thing. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.
Thermo scientific generuler 100bp dna ladder 100bp, 0. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. This marker is ideal for the size determination of digested dna. Generuler 1 kb plus dna ladder thermo fisher scientific. The 1636 bp band and all bands less than bp are generated from pbr322.
The ladder is premixed with gel load buffer color dyes with varying mobility on an agarose gel for direct transfer to the gel. Page 1 4 material safety data sheet prepared in accordance with eu directive 91155eec printed. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. Supplementary information 1 for isothermal self assembly of. Product information thermo scientific generuler 1 kb.
Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Dna ladder 100 bp 1kb marker for agarose gel electrophoresis. Phylogenetic analysis and prey identification of spiders from. The dna includes fragments ranging from 25010,000 base pairs. Do not exceed the amount of electrophoresis tracking dyes used for sample ladder preparation. Ready to load ladders contain highly purified pcr products combined with plasmid digests, they are supplied in 1x loading dye. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. Generuler 1 kb dna ladder, readytouse thermo fisher scientific. Thermo scientific generuler 1kb dna ladder, readyto use, is designed for sizing and approximate quantification of.
View more information about our dna stains technology. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. The 1 kb dna ladder was not designed for precise quantification of dna mass, but can be used for approximating the mass of dna in comparably intense samples of similar size. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder. Readytouse 1 kb dna ladder and readytouse 100 bp dna ladder. For quantification, adjust the concentration of the sample to equalize it approximately with the amount of dna in the nearest band of the ladder. The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a. Nucleic acid ladder is used as a size standard in agarose gel electrophoresis. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. The ladder is composed of fifteen chromatographypurified individual dna. Dna fragments of 100 bp with unique restriction site at both ends were self ligated to create a tandem repeat. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp.
1528 1470 253 754 563 51 529 1215 854 1479 1104 542 958 152 432 627 579 798 867 1357 764 1125 208 1461 1079 325 609 891 998 47 1215 684 767 368 275 1043 1063 1126 1445 369 1076 582 437 1154 1422 339 273 1109